[Campbell Biology P.474] In RNA sequencing RNA-seq , despite its name, what molecular compon... | Practice Question

In RNA sequencing RNA-seq , despite its name, what molecular component is actually sequenced, and what is the primary reason for fragmenting mRNA samples prior to this step?

  • A: cDNA is actually sequenced; mRNA samples are fragmented to allow for conversion into shorter, similar-sized cDNAs suitable for sequencing.
  • B: mRNA is directly sequenced; fragmentation increases the efficiency of reverse transcription.
  • C: Genomic DNA is sequenced; fragmentation prevents non-specific binding of primers.
  • D: Proteins are sequenced; fragmentation helps in separating different protein types.

Explanation

The text explicitly states: 'even though it is the cDNA that is actually sequenced' in RNA-seq, and 'In RNA-seq, the mRNA or other RNA samples are isolated, cut into shorter, similar-sized fragments, and converted into cDNAs Figure 20.12 . These short cDNA stretches are sequenced...'