[Campbell Biology P.474] What is the distinct advantage of quantitative RT-PCR qRT-PCR over ... | Practice Question
What is the distinct advantage of quantitative RT-PCR qRT-PCR over traditional RT-PCR, as mentioned in the text?
A: qRT-PCR provides quantitative data on gene expression, thereby avoiding the need for gel electrophoresis for measurement.
B: It allows for the direct sequencing of RNA molecules without prior conversion to cDNA.
C: It can identify unknown genes present in a sample more efficiently than other methods.
D: It eliminates the need for reverse transcriptase enzyme, simplifying the process.
Explanation
The text states that qRT-PCR 'can detect the light and measure the PCR product, thus avoiding the need for electrophoresis while providing quantitative data, a distinct advantage.'