[Campbell Biology P.466] What is the primary method used to detect nucleotide incorporation ... | Practice Question

What is the primary method used to detect nucleotide incorporation in Sequencing by Synthesis SBS as described in the provided text?

  • option_A: Measuring changes in pH.
  • option_B: Detecting fluorescence signals.
  • option_C: Recording flashes of light.
  • option_D: Monitoring electrical current fluctuations.

Explanation

According to step 6 of the 'Sequencing by Synthesis' method, when a complementary nucleotide is joined to the growing strand, pyrophosphate PP i is released, which causes a flash of light that is recorded. This light signal is the primary detection mechanism.