[Campbell Biology P.469] What is the correct order of the three main steps in a single cycle... | Practice Question

What is the correct order of the three main steps in a single cycle of the Polymerase Chain Reaction PCR ?

  • A: Annealing, Denaturation, Extension
  • B: Denaturation, Annealing, Extension
  • C: Extension, Denaturation, Annealing
  • D: Denaturation, Extension, Annealing

Explanation

A single PCR cycle consists of three main steps: 1 Denaturation, where the DNA is heated to separate the double strands; 2 Annealing, where the temperature is lowered to allow primers to bind to the template DNA; and 3 Extension, where DNA polymerase synthesizes new DNA strands.