[Campbell Biology P.489] Based on the provided diagram and text regarding eukaryotic gene cl... | Practice Question

Based on the provided diagram and text regarding eukaryotic gene cloning, which statement is true?

  • A: The cloning vector is usually an animal virus designed for gene delivery.
  • B: DNA fragments are ligated into the vector using a different restriction enzyme than the one used to cut the vector, to ensure compatibility.
  • C: Recombinant DNA plasmids are introduced into host cells, which then divide to form a cell clone.
  • D: Expressing cloned eukaryotic genes in bacterial host cells is a straightforward process without significant challenges.

Explanation

The diagram illustrates the process where recombinant DNA plasmids are formed and then introduced into host cells, which subsequently divide to create clones. The text confirms that recombinant plasmids are returned to host cells, each dividing to form a cell clone. Option A is incorrect because the diagram and text specify a bacterial plasmid as the cloning vector. Option B is incorrect because the diagram states the same restriction enzyme is used. Option D is incorrect as the text mentions 'several technical difficulties' in expressing eukaryotic genes in bacterial host cells.