[Campbell Biology P.425] In this DNA deletion experiment, identify the independent variable,... | Practice Question
In this DNA deletion experiment, identify the independent variable, the dependent variable, and the control treatment.
A: Independent Variable: The specific control element(s) deleted in the DNA constructs. Dependent Variable: The relative level of reporter mRNA. Control Treatment: The DNA construct with the intact enhancer region.
B: Independent Variable: The relative level of reporter mRNA. Dependent Variable: The specific control element(s) deleted. Control Treatment: The DNA construct with element 1 deleted.
C: Independent Variable: The human cell culture. Dependent Variable: The amount of DNA constructs introduced. Control Treatment: Cells not exposed to any DNA constructs.
D: Independent Variable: The approximate 8 kb of DNA region. Dependent Variable: The promoter gene. Control Treatment: The mPGES-1 gene.
Explanation
The independent variable is what the researchers manipulate, which is the specific control element s deleted in the DNA constructs elements 1, 2, or 3, or none for the control . The dependent variable is what is measured in response, which is the relative level of reporter mRNA. The control treatment is the condition where no manipulation occurs, which is the DNA construct with the intact enhancer region, serving as the baseline 100% expression .