[Campbell Biology P.144] Which light microscopy technique is described as using a laser to c... | Practice Question

Which light microscopy technique is described as using a laser to create a single plane of fluorescence, thereby eliminating out-of-focus light and enabling 3-D reconstruction?

  • A: Brightfield microscopy
  • B: Phase-contrast microscopy
  • C: Confocal microscopy
  • D: Fluorescence microscopy

Explanation

The 'Confocal' section explicitly states: 'In confocal microscopy, a laser is used to create a single plane of fluorescence; out-of-focus light from other planes is eliminated. By capturing sharp images at many different planes, a 3-D reconstruction can be created.'