[Campbell Biology P.488] Based on the provided text, which statement correctly differentiate... | Practice Question

Based on the provided text, which statement correctly differentiates between the mentioned DNA sequencing methods?

  • A: The dideoxy sequencing method is a high-throughput technique based on sequencing by synthesis.
  • B: Third-generation sequencing methods sequence short DNA molecules one at a time.
  • C: Next-generation sequencing techniques rely on DNA polymerase to synthesize a stretch of DNA, revealing the sequence.
  • D: Nucleic acid hybridization is a method used for sequencing entire genomes quickly and inexpensively.

Explanation

The text specifically states that 'Fast and inexpensive next-generation high-throughput techniques for sequencing DNA are based on sequencing by synthesis: DNA polymerase is used to synthesize a stretch of DNA using a single-stranded template, and the order in which nucleotides are added reveals the sequence.' This directly matches option C.