[Campbell Biology P.492] Which of the following best describes the fundamental principle of ... | Practice Question

Which of the following best describes the fundamental principle of the whole-genome shotgun approach to DNA sequencing?

  • A: Sequencing long DNA molecules directly without fragmentation.
  • B: Assembling overlapping short DNA sequences from randomly cut fragments using computational software.
  • C: Cloning DNA fragments sequentially based on known genetic markers.
  • D: Utilizing only dideoxy chain termination for precise single-base pair sequencing.

Explanation

The text states: 'The whole-genome shotgun approach starts with the cloning and sequencing of DNA fragments from randomly cut DNA. Powerful computer programs then assemble the resulting very large number of overlapping short sequences into a single continuous sequence Figure 21.2 .'