[Campbell Biology P.490] Which statement is true regarding cDNA produced using human brain t... | Practice Question

Which statement is true regarding cDNA produced using human brain tissue as the starting material?

  • A: The procedure to make it requires amplification by the polymerase chain reaction.
  • B: It is produced from pre-mRNA using reverse transcriptase.
  • C: It can be labeled and used as a probe to detect genes expressed in the brain.
  • D: It includes the introns of the pre-mRNA.

Explanation

cDNA is a DNA copy of mature mRNA, representing the genes that are actively expressed in a particular tissue like the brain . Because it's a complementary sequence, it can be labeled and used as a probe to identify and locate those specific mRNA sequences and thus the genes within a sample.