[Campbell Biology P.488] When creating recombinant DNA molecules, what is the correct sequen... | Practice Question

When creating recombinant DNA molecules, what is the correct sequence of events following the initial cutting of DNA by restriction enzymes?

  • A: Gel electrophoresis to separate fragments, then DNA ligase to seal.
  • B: Sticky ends base-pair with complementary sticky ends, then DNA ligase produces recombinant DNA molecules.
  • C: Polymerase chain reaction (PCR) to amplify fragments, then gel electrophoresis.
  • D: Producing multiple copies of a gene, then separating them by length.

Explanation

The text explicitly describes that after restriction enzymes cut DNA, 'The sticky ends on restriction fragments from one DNA source can base-pair with complementary sticky ends on fragments from other DNA molecules. Sealing the base-paired fragments with DNA ligase produces recombinant DNA molecules.' This sequence aligns directly with option B.