[Campbell Biology P.466] What happens in Sequencing by Synthesis if the added deoxynucleosid... | Practice Question

What happens in Sequencing by Synthesis if the added deoxynucleoside triphosphate dNTP is not complementary to the next base on the template strand?

  • option_A: It is incorporated but emits a different signal.
  • option_B: It is washed off without incorporation or light emission.
  • option_C: It causes a strong flash of light due to an error.
  • option_D: It triggers a DNA repair mechanism.

Explanation

According to step 7, if the added deoxynucleoside triphosphate dNTP is not complementary to the next base on the template strand, it is not joined to the strand, no reaction occurs, and there is no flash. It is subsequently washed off before the next dNTP is introduced.